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rb defective bladder carcinoma cell line  (ATCC)


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    ATCC rb defective bladder carcinoma cell line
    Rb Defective Bladder Carcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 992 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rb+defective+bladder+carcinoma+cell+line+5637/5637/pm18952290-24-6-11
    Average 97 stars, based on 992 article reviews
    rb defective bladder carcinoma cell line - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Generated:

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Hybridization:

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Labeling:

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Binding Assay:

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Isolation:

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Autoradiography:

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    In Vitro:

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Mutagenesis:

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation
    Article Snippet: Cell cultures.Cell cultures.. 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.. 12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.12-27 and 68.2A5 are transformed with both an Rb expression vector and a G418 resistance gene.



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    rb defective bladder carcinoma cell line  (ATCC)
    97
    ATCC rb defective bladder carcinoma cell line
    Rb Defective Bladder Carcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rb+defective+bladder+carcinoma+cell+line+5637/5637/pm18952290-24-6-11
    Average 97 stars, based on 1 article reviews
    rb defective bladder carcinoma cell line - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    rb defective bladder carcinoma cell line 5637  (ATCC)
    97
    ATCC rb defective bladder carcinoma cell line 5637
    Loss of Rb does not result in loss of DNase I-hypersensitive sites in the HLA-DRA promoter. (A) Schematic diagram of HLA-DRA promoter region and coding sequences. The previously described (17) major site of DNase I hypersensitivity in the HLA-DRA promoter is indicated as HS 1. W, W (S/Z) box; X, X1 and X2 boxes; Y, Y box; O, octamer element; α1, α2, and TM, regions of gene coding for α1, α2, and transmembrane domains of DRα, respectively. Digestion of genomic DNA with restriction endonuclease PstI generates an approximately 6.1-kb fragment encompassing the HLA-DRA promoter and most of the coding sequences. If the promoter chromatin is in a generally accessible conformation, further digestion of the genomic DNA with DNase I results in the generation of an approximately 3.7-kb fragment. The 3.7-kb cleavage fragment generated by digestion with PstI and DNase I is detected by hybridization with a radiolabeled DNA probe. (B) Rb-reconstituted (68.2A5) and Rb-defective (66.1A3) subclones of non-small-cell lung carcinoma cell line H2009 were analyzed for their sensitivity to cleavage by DNase I within the HLA-DRA promoter. HS 1 is detected in both Rb-reconstituted and Rb-defective cells and is indicated by an arrow labeled as 3.7 (HS 1). The parental PstI fragment that was not digested with DNase I is indicated by an arrow labeled as 6.1 (PstI). (C) Rb-reconstituted (12-27) and Rb-defective (1A4) subclones of bladder carcinoma cell <t>line</t> <t>5637</t> were analyzed exactly as described in panel B for their sensitivity to DNase I cleavage within the HLA-DRA promoter.
    Rb Defective Bladder Carcinoma Cell Line 5637, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rb+defective+bladder+carcinoma+cell+line+5637/5637/pmc00099796-47-7-13
    Average 97 stars, based on 1 article reviews
    rb defective bladder carcinoma cell line 5637 - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Loss of Rb does not result in loss of DNase I-hypersensitive sites in the HLA-DRA promoter. (A) Schematic diagram of HLA-DRA promoter region and coding sequences. The previously described (17) major site of DNase I hypersensitivity in the HLA-DRA promoter is indicated as HS 1. W, W (S/Z) box; X, X1 and X2 boxes; Y, Y box; O, octamer element; α1, α2, and TM, regions of gene coding for α1, α2, and transmembrane domains of DRα, respectively. Digestion of genomic DNA with restriction endonuclease PstI generates an approximately 6.1-kb fragment encompassing the HLA-DRA promoter and most of the coding sequences. If the promoter chromatin is in a generally accessible conformation, further digestion of the genomic DNA with DNase I results in the generation of an approximately 3.7-kb fragment. The 3.7-kb cleavage fragment generated by digestion with PstI and DNase I is detected by hybridization with a radiolabeled DNA probe. (B) Rb-reconstituted (68.2A5) and Rb-defective (66.1A3) subclones of non-small-cell lung carcinoma cell line H2009 were analyzed for their sensitivity to cleavage by DNase I within the HLA-DRA promoter. HS 1 is detected in both Rb-reconstituted and Rb-defective cells and is indicated by an arrow labeled as 3.7 (HS 1). The parental PstI fragment that was not digested with DNase I is indicated by an arrow labeled as 6.1 (PstI). (C) Rb-reconstituted (12-27) and Rb-defective (1A4) subclones of bladder carcinoma cell line 5637 were analyzed exactly as described in panel B for their sensitivity to DNase I cleavage within the HLA-DRA promoter.

    Journal:

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation

    doi: 10.1128/MCB.21.19.6495-6506.2001

    Figure Lengend Snippet: Loss of Rb does not result in loss of DNase I-hypersensitive sites in the HLA-DRA promoter. (A) Schematic diagram of HLA-DRA promoter region and coding sequences. The previously described (17) major site of DNase I hypersensitivity in the HLA-DRA promoter is indicated as HS 1. W, W (S/Z) box; X, X1 and X2 boxes; Y, Y box; O, octamer element; α1, α2, and TM, regions of gene coding for α1, α2, and transmembrane domains of DRα, respectively. Digestion of genomic DNA with restriction endonuclease PstI generates an approximately 6.1-kb fragment encompassing the HLA-DRA promoter and most of the coding sequences. If the promoter chromatin is in a generally accessible conformation, further digestion of the genomic DNA with DNase I results in the generation of an approximately 3.7-kb fragment. The 3.7-kb cleavage fragment generated by digestion with PstI and DNase I is detected by hybridization with a radiolabeled DNA probe. (B) Rb-reconstituted (68.2A5) and Rb-defective (66.1A3) subclones of non-small-cell lung carcinoma cell line H2009 were analyzed for their sensitivity to cleavage by DNase I within the HLA-DRA promoter. HS 1 is detected in both Rb-reconstituted and Rb-defective cells and is indicated by an arrow labeled as 3.7 (HS 1). The parental PstI fragment that was not digested with DNase I is indicated by an arrow labeled as 6.1 (PstI). (C) Rb-reconstituted (12-27) and Rb-defective (1A4) subclones of bladder carcinoma cell line 5637 were analyzed exactly as described in panel B for their sensitivity to DNase I cleavage within the HLA-DRA promoter.

    Article Snippet: 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.

    Techniques: Generated, Hybridization, Labeling

    Oct-1 facilitates repression by HDAC. (A) Oct-1 binding to the HLA-DRA octamer element is disrupted by HDAC inhibitors. EMSA were performed using nuclear protein extracts from Rb-defective bladder carcinoma cell line 5637 and its Rb-reconstituted subclone, 12-27. Each lane represents 3 μg of total nuclear protein and 25 fmol of labeled −62/−37 HLA-DRA octamer element probe. Lanes 1 and 2, extracts from 12-27 and 5637 cells, respectively; lanes 3 and 4, extracts from 5637 cells treated with 1 mM sodium butyrate and 200 nM TSA, respectively, for 72 h prior to the isolation of nuclear proteins. The upper band in the autoradiograph represents the Oct-1 protein-DNA complex, as it is supershifted by both anti-Oct-1 antibodies (Ab) (lanes 8 and 9), comigrates with in vitro-translated Oct-1 added to the binding reaction (lane 10), and is competed only by a wild-type (WT) octamer element oligonucleotide (oligo) (lane 6). The band labeled Oct-1T may represent a truncated isoform of Oct-1, as it is supershifted only by an antibody specific for the Oct-1 POU linker domain (lane 9) and not by an antibody specific for the C terminus of Oct-1 (lane 8). Apparently, the DNA binding specificity of this protein is different from that of Oct-1 as well, as it is competed by both wild-type and mutant octamer element oligonucleotides (lanes 6 and 7). (B and C) Binding of NF-Y to the HLA-DRA Y box and YY1 to the +62/+72 HLA-DRA YY1 binding element is not affected by treatment of cells with HDAC inhibitors. EMSA were performed exactly as described above, except that the HLA-DRA octamer element probe was replaced with either a Y box (B) or a YY1 binding site (C) probe.

    Journal:

    Article Title: Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation

    doi: 10.1128/MCB.21.19.6495-6506.2001

    Figure Lengend Snippet: Oct-1 facilitates repression by HDAC. (A) Oct-1 binding to the HLA-DRA octamer element is disrupted by HDAC inhibitors. EMSA were performed using nuclear protein extracts from Rb-defective bladder carcinoma cell line 5637 and its Rb-reconstituted subclone, 12-27. Each lane represents 3 μg of total nuclear protein and 25 fmol of labeled −62/−37 HLA-DRA octamer element probe. Lanes 1 and 2, extracts from 12-27 and 5637 cells, respectively; lanes 3 and 4, extracts from 5637 cells treated with 1 mM sodium butyrate and 200 nM TSA, respectively, for 72 h prior to the isolation of nuclear proteins. The upper band in the autoradiograph represents the Oct-1 protein-DNA complex, as it is supershifted by both anti-Oct-1 antibodies (Ab) (lanes 8 and 9), comigrates with in vitro-translated Oct-1 added to the binding reaction (lane 10), and is competed only by a wild-type (WT) octamer element oligonucleotide (oligo) (lane 6). The band labeled Oct-1T may represent a truncated isoform of Oct-1, as it is supershifted only by an antibody specific for the Oct-1 POU linker domain (lane 9) and not by an antibody specific for the C terminus of Oct-1 (lane 8). Apparently, the DNA binding specificity of this protein is different from that of Oct-1 as well, as it is competed by both wild-type and mutant octamer element oligonucleotides (lanes 6 and 7). (B and C) Binding of NF-Y to the HLA-DRA Y box and YY1 to the +62/+72 HLA-DRA YY1 binding element is not affected by treatment of cells with HDAC inhibitors. EMSA were performed exactly as described above, except that the HLA-DRA octamer element probe was replaced with either a Y box (B) or a YY1 binding site (C) probe.

    Article Snippet: 1A4 and 12-27 are subclones of the Rb-defective bladder carcinoma cell line 5637 (ATCC HTB9), while 66.1A3 and 68.2A5 are subclones of the parental Rb-defective non-small-cell lung carcinoma cell line H2009.

    Techniques: Binding Assay, Labeling, Isolation, Autoradiography, In Vitro, Mutagenesis